Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-O-NMA-G (iBu) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

Produkt-Nr.CH65083CAS-Nr.1025783-22-5Reinheit>=98% (HPLC)
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Produktübersicht

Kurzüberblick

Produktfamilie
Oligonucleotide Synthesis, RNA Raw Materials & Modification
MF
C47H59N8O10P
MW
927.0
Reinheit
>=98% (HPLC)

2'-O-NMA-G (iBu) CE Phosphoramidite is a 2'-modified RNA building block: 5'-O-(4,4'-dimethoxytrityl)-N2-isobutyrylguanosine with a 2'-O-[2-(methylamino)-2-oxoethyl] group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. It is identified by CAS 1025783-22-5, molecular formula C47H59N8O10P, molecular weight 927.0, PubChem CID 168441196, and InChIKey BNLNDNDXCOPBTF-NCJSJSGWSA-N.

"NMA" denotes the 2'-O-N-methylacetamide group (2'-O-CH2-C(=O)-NH-CH3), a permanent 2'-O-modification rather than a protecting group. The cited 2'-modification literature is used here as background for the 2'-position modification class, not as a standalone performance claim for this catalog item. The monomer couples through a standard 3'-phosphoramidite (3'→5' linkages), the 5'-DMT is removed each cycle, and the N2-isobutyryl base-protecting group is removed during final deprotection.

Synonyme

5'-O-DMT-N2-iBu-2'-O-[2-(methylamino)-2-oxoethyl]guanosine 3'-CE phosphoramidite; 2'-O-(N-methylcarbamoylmethyl)-guanosine amidite

Identität und Spezifikationen

Catalog No.CH65083
CAS No.1025783-22-5
Molecular FormulaC47H59N8O10P
Molecular Weight927.0
PubChem CID168441196
InChIKeyBNLNDNDXCOPBTF-NCJSJSGWSA-N
Purity>=98% (HPLC)

Lagerbedingungen

<=-20 C

Technische Merkmale

Monomer class
2'-O-NMA CE phosphoramidite
Nucleobase
Guanine
Sugar modification
2'-O-(N-methylacetamide)
Base protection
N2-isobutyryl
Packaging
1 g; 5 g; Custom packaging on request
Water content
<=0.5%
Physical form
white or off-white to faint yellow powder
Stability
stable under recommended storage conditions

Anwendungskontext

  • 2'-O-N-methylacetamide (NMA) modification: a permanent 2'-O-amide substituent at the 2'-position.
  • 2'-modification literature context: the cited literature supports the broader 2'-position modification class; avoid rewriting it as a standalone performance claim for this catalog item.
  • 3'→5' coupling monomer: the 3'-phosphoramidite couples to form 3'→5' linkages; the 5'-DMT is removed each cycle and the N2-isobutyryl base-protecting group is removed during final deprotection.

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Öffentliche Quellen

Häufig gestellte Fragen

What does 'NMA' stand for in this monomer?

NMA is the 2'-O-N-methylacetamide group — a 2'-O-CH₂-C(=O)-NH-CH₃ substituent (2'-O-[2-(methylamino)-2-oxoethyl]). It is a permanent 2'-O-modification, not a protecting group.

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