Thiol-Modifier C6 S-S Phosphoramidite
Oligonucleotide Conjugation & Delivery Ligands
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Conjugation & Delivery Ligands
- MF
- C42H61N2O5PS2
- MW
- 769.1
- Reinheit
- >=99% (HPLC)
Thiol-Modifier C6 S-S Phosphoramidite is a non-nucleoside modifier for adding a reactive thiol group to an oligonucleotide: a six-carbon linker terminating in a disulfide (S-S)-protected thiol, with a 5'-O-(4,4'-dimethoxytrityl) handle and a (2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 148254-21-1, molecular formula C42H61N2O5PS2, molecular weight 769.1, and PubChem CID 15409186.
Coupling attaches the linker to the oligonucleotide (typically the 5'-terminus). The thiol is carried as an internal disulfide during synthesis; reducing the disulfide afterward (e.g. with a thiol reducing agent) reveals a free sulphydryl group on a C6 spacer. That thiol can then be derivatised with thiol-specific probes such as maleimides or iodoacetates.
Synonyme
5'-Thiol-Modifier C6 S-S; DMT-protected hexyl disulfide thiol linker phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65106 |
| CAS No. | 148254-21-1 |
| Molecular Formula | C42H61N2O5PS2 |
| Molecular Weight | 769.1 |
| PubChem CID | 15409186 |
| InChIKey | VEONRKLBSGQZRU-UHFFFAOYSA-N |
| Purity | >=99% (HPLC) |
Lagerbedingungen
-20 C
Technische Merkmale
- Modifier type
- Non-nucleoside thiol-modifier phosphoramidite
- Spacer
- C6 (hexyl)
- Protected handle
- Disulfide-protected thiol
- Exposed handle
- Thiol after disulfide reduction
- Grade
- N (Normal)
- Packaging
- 10 mg; 25 mg; 50 mg; 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 10 kg; 25 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colorless oily liquid
- Stability
- Solution: 2-3 days; SDS: stable
Anwendungskontext
- Thiol conjugation handle: provides a thiol on a six-carbon spacer at the oligonucleotide terminus for thiol-specific coupling.
- Disulfide-protected: the thiol is carried as an S-S disulfide during synthesis and revealed by reduction afterward.
- Reacts with thiol-specific probes: the free thiol conjugates with maleimides, iodoacetates, and similar thiol-reactive reagents.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Targeted delivery of antisense oligonucleotides to hepatocytes using triantennary N-acetyl galactosamine improves potency in mice
Nucleic Acids Research, 2014
Product-family technical context
- Multivalent N-acetylgalactosamine-conjugated siRNA localizes in hepatocytes and elicits RNAi-mediated gene silencing
Journal of the American Chemical Society, 2014
Product-family technical context
- siRNA Conjugates Carrying Sequentially Assembled Trivalent N-Acetylgalactosamine Linked Through Nucleosides Elicit Robust Gene Silencing In Vivo in Hepatocytes
ACS Chemical Biology, 2015
Product-family technical context
- Comparative Characterization of Hepatic Distribution and mRNA Reduction of Antisense Oligonucleotides Conjugated with Triantennary N-Acetyl Galactosamine and Lipophilic Ligands
Journal of Pharmacology and Experimental Therapeutics, 2016
Product-family technical context
- PubChem-Verbindung 15409186
PubChem · CID 15409186
Häufig gestellte Fragen
What does the Thiol-Modifier C6 S-S do?
It provides a thiol group on a six-carbon spacer at an oligonucleotide terminus. The thiol is protected as a disulfide during synthesis; after reduction, the free thiol can be conjugated with thiol-specific probes such as maleimides or iodoacetates.
Why is the thiol supplied as a disulfide (S-S)?
A free thiol is reactive and prone to oxidation, so this modifier carries the thiol in disulfide-protected form during synthesis. Reducing the disulfide afterward reveals the free sulphydryl group for conjugation.
Ähnliche Produkte
Benötigen Sie Hilfe bei der Materialauswahl?
CHEMOS kann Produktfit, Zielstruktur, Routenfragen, analytische Erwartungen und projektspezifische Lieferbedarfe prüfen.