2',3'-Dideoxythymidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H14N2O4
- MW
- 226.23
- Reinheit
- >98.0% (HPLC, titration)
2',3'-Dideoxythymidine (ddT) is a pyrimidine 2',3'-dideoxynucleoside with thymine attached to a sugar lacking both the 2'- and 3'-hydroxyl groups. CH64018 is identified by CAS 3416-05-5, molecular formula C10H14N2O4, molecular weight 226.23, and PubChem CID 65119. It is distinct from the didehydro analog d4T.
After conversion to the corresponding triphosphate, the missing 3'-hydroxyl prevents further DNA-chain extension after polymerase incorporation. That property defines its chain-termination context in DNA-polymerase and dideoxy-sequencing research.
Synonyme
2',3'-Dideoxythymidine; ddT; 3'-deoxythymidine analog (see Identity Check)
Identität und Spezifikationen
| Catalog No. | CH64018 |
| CAS No. | 3416-05-5 |
| Molecular Formula | C10H14N2O4 |
| Molecular Weight | 226.23 |
| PubChem CID | 65119 |
| InChIKey | XKKCQTLDIPIRQD-JGVFFNPUSA-N |
| Purity | >98.0% (HPLC, titration) |
Lagerbedingungen
Room temperature; cool and dark place below 15 C; inert gas
Technische Merkmale
- Nucleobase
- Thymine (5-methyluracil)
- Sugar
- 2',3'-Dideoxyribose
- Nucleoside class
- Pyrimidine dideoxynucleoside
- Research role
- Chain-terminator nucleotide precursor
- Packaging
- 250 mg glass bottle with plastic insert; Custom packaging on request
- Physical form
- Solid; white to light yellow powder to crystal
- Stability
- >=4 years
Anwendungskontext
- Chain-terminator precursor: the corresponding ddTTP is incorporated by DNA polymerase as a chain-terminating nucleotide in dideoxy-sequencing research.
- DNA-polymerase research: ddT provides the thymidine dideoxynucleoside identity for polymerase incorporation and chain-termination studies.
- Dideoxynucleoside reference: CH64018 provides the 2',3'-dideoxythymidine identity for nucleoside chemistry comparisons.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 65119
PubChem · CID 65119
Häufig gestellte Fragen
Why is 2',3'-dideoxythymidine a chain terminator?
It lacks a 3'-hydroxyl, so once its triphosphate is incorporated by DNA polymerase no further nucleotide can be added and the chain terminates — the principle behind dideoxy sequencing.
Is ddT the same as the didehydro d4T analog?
No. CH64018 is 2',3'-dideoxythymidine; d4T is 2',3'-didehydro-2',3'-dideoxythymidine, a different compound with an added double bond.
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