5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C36H39N5O8
- MW
- 669.7
- Reinheit
- 99%
5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine is 2'-O-methylguanosine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N2-isobutyryl base-protecting group. CH64118 is identified by CAS 114745-26-5, molecular formula C36H39N5O8, molecular weight 669.7, PubChem CID 135742507, and InChIKey ISQLJOGRNUQHJX-WIFIACMTSA-N.
It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N2-isobutyryl group masks the guanine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyme
5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine; 5'-O-DMT-2'-OMe-rG(iBu); DMT-2'-OMe-rG(iBu)
Identität und Spezifikationen
| Catalog No. | CH64118 |
| CAS No. | 114745-26-5 |
| Molecular Formula | C36H39N5O8 |
| Molecular Weight | 669.7 |
| PubChem CID | 135742507 |
| InChIKey | ISQLJOGRNUQHJX-WIFIACMTSA-N |
| Purity | 99% |
Lagerbedingungen
-20 C
Technische Merkmale
- Nucleobase
- Guanine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 25 mg; 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
- Stability
- 3 years at -20 C; 2 years at 4 C
Anwendungskontext
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-OMe modification context: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 135742507
PubChem · CID 135742507
Häufig gestellte Fragen
How does it differ from N2-isobutyryl-2'-O-methylguanosine?
Both carry an N2-isobutyryl-protected 2'-O-methylguanosine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.
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