2'-OMe-Cytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C42H52N5O9P
- MW
- 801.9
- Reinheit
- >=99% (NMR)
2'-OMe-Cytidine (Ac) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-acetyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65018 is identified by CAS 199593-09-4, molecular formula C42H52N5O9P, molecular weight 801.9, PubChem CID 66764086, and InChIKey WNWUMIPFLOKTEZ-UAQIPLLRSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyme
DMT-2'-OMe-rC(Ac) phosphoramidite; 5'-O-DMT-N4-acetyl-2'-O-methylcytidine 3'-CE phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65018 |
| CAS No. | 199593-09-4 |
| Molecular Formula | C42H52N5O9P |
| Molecular Weight | 801.9 |
| PubChem CID | 66764086 |
| InChIKey | WNWUMIPFLOKTEZ-UAQIPLLRSA-N |
| Purity | >=99% (NMR) |
Lagerbedingungen
-20 C; under inert atmosphere
Technische Merkmale
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Acetyl
- Grade
- Standard; TheraPure
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 100 g; Custom packaging on request
- Water content
- <=0.3 wt.% by Karl Fischer
- Physical form
- white to off-white powder
- Stability
- >=4 years
Anwendungskontext
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- 5'-DMT + N4-acetyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 66764086
PubChem · CID 66764086
Häufig gestellte Fragen
How does it differ from the 2'-OMe-C(Bz) phosphoramidite?
Both are 2'-O-methyl cytidine amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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