2'-OMe-Guanosine (dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C44H55N8O8P
- MW
- 854.9
- Reinheit
- >=98.5% (HPLC)
2'-OMe-Guanosine (dmf) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-dimethylformamidine-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65021 is identified by CAS 128219-77-2, molecular formula C44H55N8O8P, molecular weight 854.9, PubChem CID 135742510, and InChIKey ZGDBCILCTOHNAB-OWKWDSSOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyme
DMT-2'-OMe-rG(dmf) phosphoramidite; 5'-O-DMT-N2-dmf-2'-O-methylguanosine 3'-CE phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65021 |
| CAS No. | 128219-77-2 |
| Molecular Formula | C44H55N8O8P |
| Molecular Weight | 854.9 |
| PubChem CID | 135742510 |
| InChIKey | ZGDBCILCTOHNAB-OWKWDSSOSA-N |
| Purity | >=98.5% (HPLC) |
Lagerbedingungen
-20 C
Technische Merkmale
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Dimethylaminomethylene (dmf)
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Anwendungskontext
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 135742510
PubChem · CID 135742510
Häufig gestellte Fragen
What does this 2'-OMe amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection. The product provides a 2'-O-methylguanosine phosphoramidite identity.
How does it differ from the 2'-OMe-G(iBu) phosphoramidite?
Both are 2'-O-methyl guanosine amidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
Ähnliche Produkte
Benötigen Sie Hilfe bei der Materialauswahl?
CHEMOS kann Produktfit, Zielstruktur, Routenfragen, analytische Erwartungen und projektspezifische Lieferbedarfe prüfen.