2'-OMe-Cytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C47H54N5O9P
- MW
- 863.9
- Reinheit
- >99.00%
2'-OMe-Cytidine (Bz) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65023 is identified by CAS 110764-78-8, molecular formula C47H54N5O9P, molecular weight 863.9, PubChem CID 13872225, and InChIKey JFFSFQRVIPPCBC-VKBHKTMGSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyme
DMT-2'-OMe-rC(Bz) phosphoramidite; 5'-O-DMT-N4-benzoyl-2'-O-methylcytidine 3'-CE phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65023 |
| CAS No. | 110764-78-8 |
| Molecular Formula | C47H54N5O9P |
| Molecular Weight | 863.9 |
| PubChem CID | 13872225 |
| InChIKey | JFFSFQRVIPPCBC-VKBHKTMGSA-N |
| Purity | >99.00% |
Lagerbedingungen
-20 C
Technische Merkmale
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Benzoyl
- Grade
- N (Normal)
- Packaging
- 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 25 g; 100 g; Custom packaging on request
- Physical form
- white powder
- Stability
- >=4 years
Anwendungskontext
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N4-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 13872225
PubChem · CID 13872225
Häufig gestellte Fragen
What does this 2'-OMe amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-benzoyl removed at final deprotection. The product provides a 2'-O-methylcytidine phosphoramidite identity.
How does it differ from the 2'-OMe-C(Ac) phosphoramidite?
Both are 2'-O-methyl cytidine amidites, but this one uses an N4-benzoyl base protection rather than acetyl. The acetyl amidite is cataloged separately.
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