2'-O-MOE-Cytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C49H58N5O10P
- MW
- 908.0
- Reinheit
- >=98.0% (HPLC)
2'-O-MOE-Cytidine (Bz) CE Phosphoramidite is the 2'-O-methoxyethyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-N4-benzoylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65037 is identified by CAS 251647-54-8, molecular formula C49H58N5O10P, molecular weight 908.0, PubChem CID 127262586, and InChIKey IAFVTVZPQIFRAU-NXPFYNPLSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyme
DMT-2'-O-MOE-rC(Bz) phosphoramidite; 5'-O-DMT-2'-O-MOE-N4-benzoylcytidine 3'-CE phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65037 |
| CAS No. | 251647-54-8 |
| Molecular Formula | C49H58N5O10P |
| Molecular Weight | 908.0 |
| PubChem CID | 127262586 |
| InChIKey | IAFVTVZPQIFRAU-NXPFYNPLSA-N |
| Purity | >=98.0% (HPLC) |
Lagerbedingungen
-20 C
Technische Merkmale
- Monomer class
- 2'-O-MOE RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- N4-Benzoyl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Anwendungskontext
- 2'-MOE RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies and as gapmer flank residues.
- 5'-DMT + N4-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 127262586
PubChem · CID 127262586
Häufig gestellte Fragen
What does this 2'-MOE amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-benzoyl removed at final deprotection. The product provides a 2'-O-methoxyethyl cytidine phosphoramidite identity.
How does it differ from the 2'-O-MOE-5-Me-C(Bz) phosphoramidite?
Both are N4-benzoyl-protected 2'-MOE cytidine amidites, but that one has a 5-methylcytosine base while this one has a plain cytosine base. The 5-methyl form is cataloged separately.
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