dTTP, 100 mM Solution
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H14N2Na3O14P3
- MW
- 548.11
- Reinheit
- 99.98%
dTTP, 100 mM Solution is thymidine-5'-triphosphate (dTTP, also written TTP) listed as a 100 mM aqueous solution of the trisodium salt. Key identifiers include CAS 27821-54-1, molecular formula C10H14N2Na3O14P3, and molecular weight 548.11 (PubChem CID 5311491).
dTTP is one of the four canonical deoxynucleoside triphosphates (dNTPs). DNA polymerases catalyze the template-directed polymerization of dNTP substrates onto the 3'-end of a growing DNA primer strand, so dTTP supplies the thymine (T) residue during enzymatic DNA synthesis — for example in PCR, primer extension, DNA sequencing, and nick translation.
Synonyme
thymidine 5'-triphosphate trisodium salt; TTP; dTTP·3Na, 100 mM aqueous solution
Identität und Spezifikationen
| Catalog No. | CH66004 |
| CAS No. | 27821-54-1 |
| Molecular Formula | C10H14N2Na3O14P3 (trisodium salt) |
| Molecular Weight | 548.11 |
| PubChem CID | 5311491 |
| InChIKey | KNVJTOALWGZWAI-SPSULGLQSA-K |
| Concentration | 100 mM aqueous solution |
| Purity | 99.98% |
Lagerbedingungen
-20 C
Technische Merkmale
- Nucleotide class
- dNTP
- Nucleobase
- Thymine
- 5'-phosphate state
- Triphosphate
- Grade
- PCR Grade
- Packaging
- 100 µL; 200 µL; 500 µL; 1 mL; 5 mL; 10 mL; 50 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- colorless transparent solution
- Stability
- 2 years at -20 C as a solution
Anwendungskontext
- Thymine dNTP substrate: supplies the thymine (T) residue during enzymatic, template-directed DNA synthesis by DNA polymerases.
- Common reactions: PCR, primer extension, DNA sequencing, nick translation, and other polymerase-based DNA synthesis reactions.
- Solution presentation: listed as a 100 mM aqueous solution of the trisodium salt.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 5311491
PubChem · CID 5311491
Häufig gestellte Fragen
What is dTTP used for?
dTTP (thymidine triphosphate) is the thymine deoxynucleoside triphosphate. DNA polymerases use it as a substrate to add thymine (T) residues during enzymatic, template-directed DNA synthesis — for example in PCR, primer extension, sequencing, and nick translation.
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