5-aa-2'-dUTP (Aminoallyl-dUTP)
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Reinheit
- >=98%
5-aa-2'-dUTP is 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate, commonly described as aminoallyl-dUTP. It is a modified deoxynucleotide triphosphate that carries a primary amine on a 5-aminoallyl linker at the C5 position of uracil. Key identifiers for this catalog entry are CHEMOS CH66011 and CAS 936327-10-5.
In enzymatic DNA labeling workflows, aminoallyl-dUTP can be incorporated by DNA polymerases during template-directed DNA synthesis. The introduced primary amine can then react with amine-reactive reporters such as NHS-ester dyes, allowing post-synthetic labeling of DNA products. This page keeps the identity boundary to the supplier catalog name, CAS number, and literature-supported aminoallyl-dUTP use context.
Synonyme
aminoallyl-dUTP; 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate; AA-dUTP
Identität und Spezifikationen
| Catalog No. | CH66011 |
| Product Name | 5-aa-2'-dUTP (Aminoallyl-dUTP) |
| CAS No. | 936327-10-5 |
| Product Type | Amine-modified deoxynucleotide triphosphate |
| Labeling Handle | 5-aminoallyl primary amine |
| Purity | >=98% |
Lagerbedingungen
freeze below -15 C; minimize light exposure
Technische Merkmale
- Nucleotide class
- dNTP
- Parent nucleotide
- dUTP
- Base modification
- 5-(3-Aminoallyl)
- Reactive handle
- Primary amine
- Packaging
- 1 mg; 5 mg; 25 mg; 50 mg; 100 mg; 5 x 1 mg; Custom packaging on request
- Solution concentration
- 4 mM
- Physical form
- colorless liquid
- Stability
- 6 months upon receiving
Anwendungskontext
- Amine handle in DNA: used as an aminoallyl-modified dUTP to introduce a primary amine into enzymatically synthesized DNA.
- Post-synthetic labeling: the aminoallyl handle can react with amine-reactive reporters such as NHS-ester dyes.
- Probe preparation: relevant to DNA labeling workflows such as PCR, reverse-transcription labeling, and microarray probe preparation.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Aminoallyl-dUTP sodium salt, catalog A0410
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
Häufig gestellte Fragen
What is 5-aa-2'-dUTP used for?
5-aa-2'-dUTP is an aminoallyl-modified dUTP used to introduce a primary amine into enzymatically synthesized DNA for post-synthetic labeling.
Why use an aminoallyl handle?
The aminoallyl group provides a small primary amine handle that can react with amine-reactive reporters such as NHS-ester dyes after nucleotide incorporation.
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