3'-O-allyl-2'-dATP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Reinheit
- >=98%
3'-O-allyl-2'-dATP is a 3'-O-allyl modified dATP reversible terminator. The 3'-hydroxyl is capped with a reversible blocking group so that polymerase extension stops after a single incorporation cycle. Key identifiers are CHEMOS CH66022, CAS 917766-44-0, molecular formula C13H20N5O12P3, and molecular weight 531.25.
3'-O-modified nucleotides are used in cyclic reversible-terminator sequencing-by-synthesis workflows: DNA polymerase incorporates the nucleotide, extension pauses after one base, and cap removal regenerates the 3'-OH so synthesis can continue.
Synonyme
3'-O-allyl-dATP; 3'-O-(prop-2-en-1-yl)-2'-deoxyadenosine-5'-triphosphate
Identität und Spezifikationen
| Catalog No. | CH66022 |
| Product Name | 3'-O-allyl-2'-dATP |
| CAS No. | 917766-44-0 |
| Molecular Formula | C13H20N5O12P3 |
| Molecular Weight | 531.25 |
| Product Type | 3'-O-allyl reversible-terminator dATP |
| Base Added | Adenine |
| Purity | >=98% |
Technische Merkmale
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dATP
- 3'-O blocking group
- Allyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- Custom packaging on request
Anwendungskontext
- Reversible terminator nucleotide: used as the adenine member of a 3'-O-blocked dNTP set.
- Sequencing-by-synthesis context: incorporation pauses extension for one cycle before cap removal allows synthesis to continue.
- Modified nucleotide cataloging: relevant to oligonucleotide synthesis, sequencing reagent, and modified dNTP product organization.
Zugehörige technische Ressourcen
Öffentliche Quellen
- 3'-O-Allyl-dATP, catalog HY-185480
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
Häufig gestellte Fragen
What is 3'-O-allyl-2'-dATP?
3'-O-allyl-2'-dATP is a 3'-O-allyl modified dATP used as a reversible-terminator nucleotide in sequencing-by-synthesis research workflows.
How does a reversible terminator work?
DNA polymerase incorporates the nucleotide, the 3'-O blocking group pauses further extension, and removal of the cap regenerates the 3'-OH so synthesis can continue.
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