3'-O-NH2-2'-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H18N3O14P3
- MW
- 497.18
- Reinheit
- 99.00%
3'-O-NH2-2'-dTTP is 3'-O-amino-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. Key identifiers include CAS 1159990-37-0, molecular formula C10H18N3O14P3, and molecular weight 497.18 (PubChem CID 86760875).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-amino cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyme
3'-O-amino-dTTP; 3'-O-(aminooxy)-thymidine-5'-triphosphate
Identität und Spezifikationen
| Catalog No. | CH66029 |
| CAS No. | 1159990-37-0 |
| Molecular Formula | C10H18N3O14P3 |
| Molecular Weight | 497.18 |
| PubChem CID | 86760875 |
| InChIKey | WTXJWVBIEUJGOO-XLPZGREQSA-N |
| Purity | 99.00% |
Lagerbedingungen
material in solvent at -80 C
Technische Merkmale
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Amino
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 100 µL; 500 µL; 1 mL; 5 mL; 10 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- liquid; colorless to light yellow
- Stability
- 1 year in solvent at -80 C
Anwendungskontext
- Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-amino cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Thymine building block: supplies the thymine reversible terminator, completing the four-nucleotide (A/C/G/T) 3'-O-amino SBS set.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 86760875
PubChem · CID 86760875
Häufig gestellte Fragen
What is a 3'-O-amino reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the 3'-O-amino cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the thymine (T) reversible terminator — 3'-O-amino-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-amino terminators it forms a four-nucleotide set for sequencing-by-synthesis.
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