3'-O-Ac-2'-dGTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C12H18N5O14P3
- MW
- 549.22
- Reinheit
- >95% (HPLC)
3'-O-Ac-2'-dGTP is 3'-O-acetyl-2'-deoxyguanosine-5'-triphosphate: a reversible-terminator dGTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. Key identifiers include CAS 90290-77-0, molecular formula C12H18N5O14P3, and molecular weight 549.22 (PubChem CID 172873686).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-acetyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyme
3'-O-acetyl-dGTP; 3'-O-(acetyl)-2'-deoxyguanosine-5'-triphosphate
Identität und Spezifikationen
| Catalog No. | CH66032 |
| CAS No. | 90290-77-0 |
| Molecular Formula | C12H18N5O14P3 |
| Molecular Weight | 549.22 |
| PubChem CID | 172873686 |
| InChIKey | VWTYSCJILHVGIH-XLPZGREQSA-N |
| Purity | >95% (HPLC) |
Lagerbedingungen
-70 C
Technische Merkmale
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dGTP
- 3'-O blocking group
- Acetyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 10 mg; 50 mg; 100 mg; 250 mg; 5 umol (~2.7 mg) in 500 uL vial; Custom packaging on request
- Solution concentration
- 10 mM
- Physical form
- 10 mM aqueous sodium-salt solution
- Stability
- slowly decomposes at ambient temperature; thaw only before use and avoid repeated freeze/thaw cycles
Anwendungskontext
- Reversible terminator: DNA polymerase incorporates it, adding one guanine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-acetyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Guanine building block: supplies the guanine reversible terminator in a four-nucleotide SBS set.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 172873686
PubChem · CID 172873686
Häufig gestellte Fragen
What is a 3'-O-acetyl reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the acetyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the guanine (G) reversible terminator — 3'-O-acetyl-2'-deoxyguanosine-5'-triphosphate.
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