Chemical Phosphorylation Reagent
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C34H45N2O7PS
- MW
- 656.8
- Reinheit
- 98% (HPLC)
Chemical Phosphorylation Reagent is a non-nucleoside building block for introducing a phosphate group on an oligonucleotide during synthesis: a phosphoramidite carrying a protected phosphate (with a sulfonyl-type, base-labile protecting group and a 5'-O-(4,4'-dimethoxytrityl) handle) plus a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 108783-02-4, molecular formula C34H45N2O7PS, molecular weight 656.8, and PubChem CID 10283145.
It couples at the oligonucleotide terminus like any amidite; after the phosphate protecting group is removed during deprotection, it leaves a phosphate monoester — for example a 5'-phosphate. This provides a terminally phosphorylated oligonucleotide chemically, without an enzymatic phosphorylation step.
Synonyme
CPR; Chemical Phosphorylation Reagent I; 5'-phosphate-ON reagent (sulfonyl-protected phosphate amidite)
Identität und Spezifikationen
| Catalog No. | CH65101 |
| CAS No. | 108783-02-4 |
| Molecular Formula | C34H45N2O7PS |
| Molecular Weight | 656.8 |
| PubChem CID | 10283145 |
| InChIKey | RJDYWMSSNMDIOO-UHFFFAOYSA-N |
| Purity | 98% (HPLC) |
Lagerbedingungen
-20 C
Technische Merkmale
- Reagent class
- Non-nucleoside phosphorylation phosphoramidite
- Installation site
- Oligonucleotide terminus
- Installed group
- Phosphate monoester after deprotection
- Structure distinction
- Sulfur-containing protected-phosphate structure
- Grade
- N (Normal)
- Packaging
- 1 mg; 5 mg; 10 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 5 kg; 10 kg; 25 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colourless to light brown-yellow transparent oily substance
- Stability
- prepared LK2101 solution: use within 24 h
Anwendungskontext
- Chemical Phosphorylation Reagent reference: use this chemical phosphorylation reagent record for oligonucleotide phosphorylation reagent catalog work.
- Identifier cross-check: compare CAS 108783-02-4, PubChem CID 10283145, formula C34H45N2O7PS, MW 656.8, and the listed InChIKey for catalog identity matching.
- Inquiry preparation: include catalog number CH65101, the product name, and the identity-matching identifiers when preparing a CHEMOS product inquiry.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 10283145
PubChem · CID 10283145
Häufig gestellte Fragen
What does a Chemical Phosphorylation Reagent do?
It provides a phosphate group on an oligonucleotide chemically, during solid-phase synthesis, instead of using an enzyme (kinase). It couples like an amidite and, after deprotection, leaves a phosphate monoester — for example a 5'-phosphate.
How does it fit into the synthesis cycle?
It carries a 3'-phosphoramidite and a 5'-DMT handle, so it couples in the normal amidite cycle at the position where the phosphate is wanted. Its phosphate protecting group is removed at final deprotection to reveal the terminal phosphate.
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