5'-O-DMT-2'-O-TBDMS-N6-methyladenosine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C38H47N5O6Si
- PM
- 697.9
- Pureté
- >=98.5%
5'-O-DMT-2'-O-TBDMS-N6-methyladenosine is N6-methyladenosine (m6A) carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and a 2'-O-(tert-butyldimethylsilyl) (TBDMS) group. CH64114 is identified by CAS 588698-75-3, molecular formula C38H47N5O6Si, molecular weight 697.9, PubChem CID 12994119, and InChIKey KEEBYCOQGORZDK-QGGAYTEESA-N.
It is an RNA phosphoramidite precursor context for m6A-modified RNA synthesis research: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during RNA synthesis. The N6-methyl group is the permanent m6A base modification, not a removable protecting group.
Synonymes
5'-O-DMT-2'-O-TBDMS-N6-methyladenosine; 5'-O-DMT-2'-O-TBDMS-m6A; DMT-2'-TBDMS-m6A
Identité et spécifications
| Catalog No. | CH64114 |
| CAS No. | 588698-75-3 |
| Molecular Formula | C38H47N5O6Si |
| Molecular Weight | 697.9 |
| PubChem CID | 12994119 |
| InChIKey | KEEBYCOQGORZDK-QGGAYTEESA-N |
| Purity | >=98.5% |
Conditions de stockage
2-8 C
Caractéristiques techniques
- Nucleobase
- Adenine
- 5'-O protection
- DMT (dimethoxytrityl)
- 2'-O protection
- TBDMS (tert-butyldimethylsilyl)
- Base modification
- N6-Methyl (m6A)
- Packaging
- 100 mg; 250 mg; 1 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
Contexte d’application
- m6A-modified RNA synthesis research: the base is N6-methyladenosine (m6A), treated here as a permanent base modification rather than a protecting group.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-O-TBDMS 2'-OH protection: the tert-butyldimethylsilyl group protects the ribose 2'-hydroxyl during solid-phase RNA synthesis.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 12994119
PubChem · CID 12994119
Questions fréquentes
Is the N6-methyl group a protecting group?
No. The N6-methyl is the permanent m6A base modification. Only the 5'-hydroxyl (acid-labile DMT, removed each cycle) and the ribose 2'-hydroxyl (TBDMS, for RNA synthesis) carry protecting groups.
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