5'-O-DMT-2'-O-methyluridine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C31H32N2O8
- PM
- 560.6
- Pureté
- 99.8% (HPLC)
5'-O-DMT-2'-O-methyluridine is 2'-O-methyluridine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group. CH64120 is identified by CAS 103285-22-9, molecular formula C31H32N2O8, molecular weight 560.6, PubChem CID 11039002, and InChIKey MFDHAVFJDSRPKC-YXINZVNLSA-N.
It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity. Because uracil has no exocyclic amine, no base protecting group is required.
Synonymes
5'-O-DMT-2'-O-methyluridine; 5'-O-DMT-2'-OMe-rU; DMT-2'-OMe-rU
Identité et spécifications
| Catalog No. | CH64120 |
| CAS No. | 103285-22-9 |
| Molecular Formula | C31H32N2O8 |
| Molecular Weight | 560.6 |
| PubChem CID | 11039002 |
| InChIKey | MFDHAVFJDSRPKC-YXINZVNLSA-N |
| Purity | 99.8% (HPLC) |
Conditions de stockage
0-8 C
Caractéristiques techniques
- Nucleobase
- Uracil
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-Methyl
- Base protection
- Unprotected
- Packaging
- 5 mg; 10 mg; 25 mg; 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 1 mL; Custom packaging on request
- Solution concentration
- 10 mM in DMSO
- Water content
- <=2% Karl Fischer
- Physical form
- white to off-white to faint yellow powder
- Stability
- Powder: 3 years at -20 C; in solvent: 1 year at -80 C
Contexte d’application
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- No base protection needed: uracil has no exocyclic amine, so this building block requires no base protecting group.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 11039002
PubChem · CID 11039002
Questions fréquentes
How does it differ from 5'-O-DMT-2'-O-TBDMS-uridine?
Both are 5'-O-DMT-protected uridines, but this one has a 2'-O-methyl modification (a permanent methyl on the 2'-oxygen), whereas the 2'-O-TBDMS form protects the 2'-hydroxyl with a silyl group that is later removed. The 2'-O-TBDMS form is cataloged separately.
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