2'-Deoxycytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C41H50N5O8P
- PM
- 771.8
- Pureté
- 100% (NMR)
2'-Deoxycytidine (Ac) CE Phosphoramidite is the fully protected 2'-deoxycytidine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-acetyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65005 is identified by CAS 154110-40-4, molecular formula C41H50N5O8P, molecular weight 771.8, PubChem CID 70700205, and InChIKey MECWEBCHRKVTNV-RLWDVSNXSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the N4-acetyl base group is removed at final deprotection.
Synonymes
DMT-dC(Ac) phosphoramidite; N4-acetyl-5'-O-DMT-2'-deoxycytidine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65005 |
| CAS No. | 154110-40-4 |
| Molecular Formula | C41H50N5O8P |
| Molecular Weight | 771.8 |
| PubChem CID | 70700205 |
| InChIKey | MECWEBCHRKVTNV-RLWDVSNXSA-N |
| Purity | 100% (NMR) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Cytosine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- N4-Acetyl
- Grade
- Standard; TheraPure
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; 500 g; 1 kg; 6 x 10 g; 6 x 20 g; 6 x 5 g; Custom packaging on request
- Water content
- <=0.3% w/w
- Physical form
- white to yellow powder
- Stability
- Specification print date 2026-01-01
Contexte d’application
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 70700205
PubChem · CID 70700205
Questions fréquentes
What makes this a phosphoramidite building block?
It carries a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group — the reactive coupling group used in automated solid-phase DNA synthesis. It also has an acid-labile 5'-O-DMT group (removed each cycle) and an N4-acetyl base protection (removed at final deprotection).
How does it differ from 2'-deoxycytidine (Bz) phosphoramidite?
Both are 5'-O-DMT 2'-deoxycytidine phosphoramidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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