2'-Deoxyuridine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C39H47N4O8P
- PM
- 730.8
- Pureté
- 99.75% (HPLC)
2'-Deoxyuridine CE Phosphoramidite is the 2'-deoxyuridine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65007 is identified by CAS 109389-30-2, molecular formula C39H47N4O8P, molecular weight 730.8, PubChem CID 11061591, and InChIKey ZGJUDTLSWZPIDW-MVFNBKNKSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, and the acid-labile 5'-DMT is removed at the start of each cycle. Because uracil has no exocyclic amine, no base protecting group is required.
Synonymes
DMT-dU phosphoramidite; 5'-O-DMT-2'-deoxyuridine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65007 |
| CAS No. | 109389-30-2 |
| Molecular Formula | C39H47N4O8P |
| Molecular Weight | 730.8 |
| PubChem CID | 11061591 |
| InChIKey | ZGJUDTLSWZPIDW-MVFNBKNKSA-N |
| Purity | 99.75% (HPLC) |
Conditions de stockage
-20 C, dry inert atmosphere
Caractéristiques techniques
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Uracil
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 1 mL; Custom packaging on request
- Solution concentration
- 10 mM in DMSO (supplied 1 mL solution option)
- Water content
- K.F. <=0.20% w/w
- Physical form
- white to off-white powder
- Stability
- powder: 3 years; supplied solution: 1 year
Contexte d’application
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- No base protection needed: uracil has no exocyclic amine, so this monomer requires no base protecting group.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 11061591
PubChem · CID 11061591
Questions fréquentes
Why does the 2'-deoxyuridine phosphoramidite have no base protecting group?
Uracil has no reactive exocyclic amine, so it needs no base protection. The monomer carries only the acid-labile 5'-O-DMT group (removed each cycle) and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-deoxyuridine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The 5'-O-DMT-2'-deoxyuridine precursor is cataloged separately.
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