2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C47H64N5O9PSi
- PM
- 902.1
- Pureté
- 100% (HPLC)
2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite is the fully protected cytidine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-acetylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65011 is identified by CAS 121058-88-6, molecular formula C47H64N5O9PSi, molecular weight 902.1, PubChem CID 11814945, and InChIKey QKWKXYVKGFKODW-YOEDQOPESA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, the 2'-O-TBDMS protects the ribose 2'-hydroxyl, and the N4-acetyl base group is removed at final deprotection.
Synonymes
DMT-2'-O-TBDMS-rC(Ac) phosphoramidite; 5'-O-DMT-2'-O-TBDMS-N4-acetylcytidine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65011 |
| CAS No. | 121058-88-6 |
| Molecular Formula | C47H64N5O9PSi |
| Molecular Weight | 902.1 |
| PubChem CID | 11814945 |
| InChIKey | QKWKXYVKGFKODW-YOEDQOPESA-N |
| Purity | 100% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- RNA CE phosphoramidite
- Nucleobase
- Cytosine
- 2'-O protection
- TBDMS (tert-butyldimethylsilyl)
- Base protection
- N4-Acetyl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.3% (same-SKU COA specification)
- Physical form
- white to light yellow powder
- Stability
- Manufactured 2022-09-12; retest 2026-03-15; expiry 2026-09-11
Contexte d’application
- RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
- 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
- N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 11814945
PubChem · CID 11814945
Questions fréquentes
What are the four functional groups on this RNA amidite?
A 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite (the reactive coupling group), an acid-labile 5'-O-DMT group (removed each cycle), a 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and an N4-acetyl base protection (removed at final deprotection).
How does it differ from the 2'-O-TBDMS-rC(Bz) phosphoramidite?
Both are 2'-O-TBDMS cytidine RNA amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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