2'-OMe-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C45H56N7O9P
- PM
- 869.9
- Pureté
- 100% (NMR)
2'-OMe-Guanosine (iBu) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-isobutyryl-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65019 is identified by CAS 150780-67-9, molecular formula C45H56N7O9P, molecular weight 869.9, PubChem CID 135587728, and InChIKey IRRDHRZUOZNWDJ-MLLDKZSOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonymes
DMT-2'-OMe-rG(iBu) phosphoramidite; 5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65019 |
| CAS No. | 150780-67-9 |
| Molecular Formula | C45H56N7O9P |
| Molecular Weight | 869.9 |
| PubChem CID | 135587728 |
| InChIKey | IRRDHRZUOZNWDJ-MLLDKZSOSA-N |
| Purity | 100% (NMR) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- Manufactured 2019-08-16; retest 2023-02-16; expired 2023-08-15
Contexte d’application
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 135587728
PubChem · CID 135587728
Questions fréquentes
How does it relate to 5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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