2'-OMe-Guanosine (dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C44H55N8O8P
- PM
- 854.9
- Pureté
- >=98.5% (HPLC)
2'-OMe-Guanosine (dmf) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-dimethylformamidine-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65021 is identified by CAS 128219-77-2, molecular formula C44H55N8O8P, molecular weight 854.9, PubChem CID 135742510, and InChIKey ZGDBCILCTOHNAB-OWKWDSSOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonymes
DMT-2'-OMe-rG(dmf) phosphoramidite; 5'-O-DMT-N2-dmf-2'-O-methylguanosine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65021 |
| CAS No. | 128219-77-2 |
| Molecular Formula | C44H55N8O8P |
| Molecular Weight | 854.9 |
| PubChem CID | 135742510 |
| InChIKey | ZGDBCILCTOHNAB-OWKWDSSOSA-N |
| Purity | >=98.5% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Dimethylaminomethylene (dmf)
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Contexte d’application
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 135742510
PubChem · CID 135742510
Questions fréquentes
What does this 2'-OMe amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection. The product provides a 2'-O-methylguanosine phosphoramidite identity.
How does it differ from the 2'-OMe-G(iBu) phosphoramidite?
Both are 2'-O-methyl guanosine amidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
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