2'-OMe-5-Methylcytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C48H56N5O9P
- PM
- 878.0
- Pureté
- >=98.0% (HPLC)
2'-OMe-5-Methylcytidine (Bz) CE Phosphoramidite is the 2'-O-methyl 5-methylcytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-O-methyl-5-methylcytidine (5-methylcytosine base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65024 is identified by CAS 166593-57-3, molecular formula C48H56N5O9P, molecular weight 878.0, PubChem CID 11967352, and InChIKey GIWYLFDPXKHBKT-AENBYTAOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonymes
DMT-2'-OMe-5Me-rC(Bz) phosphoramidite; 5'-O-DMT-N4-benzoyl-2'-O-methyl-5-methylcytidine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65024 |
| CAS No. | 166593-57-3 |
| Molecular Formula | C48H56N5O9P |
| Molecular Weight | 878.0 |
| PubChem CID | 11967352 |
| InChIKey | GIWYLFDPXKHBKT-AENBYTAOSA-N |
| Purity | >=98.0% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- 5-Methylcytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Benzoyl
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; Custom packaging on request
- Physical form
- white to off-white powder
- Stability
- powder: 3 years at -20 C; 2 years at 4 C
Contexte d’application
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5-Methylcytosine base, N4-benzoyl protected: the base is 5-methylcytosine with an N4-benzoyl protecting group (removed at final deprotection), and the acid-labile 5'-DMT is removed each cycle.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 11967352
PubChem · CID 11967352
Questions fréquentes
What base and sugar identity does this amidite provide?
It provides a 2'-O-methyl-5-methylcytidine phosphoramidite identity with a 5-methylcytosine base. The 3'-phosphoramidite is the coupling group, 5'-DMT is removed each cycle, and N4-benzoyl is removed at final deprotection.
How does it differ from the 2'-OMe-C(Bz) phosphoramidite?
Both are N4-benzoyl-protected 2'-O-methyl cytidine amidites, but this one has a 5-methylcytosine base (an extra methyl on the base). The non-methylated form is cataloged separately.
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