2'-F-Cytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C46H51FN5O8P
- PM
- 851.9
- Pureté
- >98.5% (HPLC)
2'-F-Cytidine (Bz) CE Phosphoramidite is the 2'-fluoro cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N4-benzoyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65027 is identified by CAS 161442-19-9, molecular formula C46H51FN5O8P, molecular weight 851.9, PubChem CID 10328204, and InChIKey CKKJPMGSTGVCJJ-GNUWXFRUSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
Synonymes
DMT-2'-F-rC(Bz) phosphoramidite; 5'-O-DMT-2'-fluoro-N4-benzoyl-2'-deoxycytidine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65027 |
| CAS No. | 161442-19-9 |
| Molecular Formula | C46H51FN5O8P |
| Molecular Weight | 851.9 |
| PubChem CID | 10328204 |
| InChIKey | CKKJPMGSTGVCJJ-GNUWXFRUSA-N |
| Purity | >98.5% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-fluoro RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N4-Benzoyl
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 100 g; 500 g; Custom packaging on request
- Physical form
- white to off-white powder
- Stability
- stable under recommended storage conditions
Contexte d’application
- 2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- 5'-DMT + N4-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 10328204
PubChem · CID 10328204
Questions fréquentes
What does this 2'-F amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-benzoyl removed at final deprotection. The product provides a 2'-fluoro cytidine phosphoramidite identity.
How does it relate to 5'-O-DMT-2'-fluoro-N4-benzoyl-2'-deoxycytidine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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