2'-F-Cytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C41H49FN5O8P
- PM
- 789.8
- Pureté
- 100% (HPLC)
2'-F-Cytidine (Ac) CE Phosphoramidite is the 2'-fluoro cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N4-acetyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65030 is identified by CAS 159414-99-0, molecular formula C41H49FN5O8P, molecular weight 789.8, PubChem CID 67090252, and InChIKey CNFKJHKDSRXNFL-UTXREMQHSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
Synonymes
DMT-2'-F-rC(Ac) phosphoramidite; 5'-O-DMT-2'-fluoro-N4-acetyl-2'-deoxycytidine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65030 |
| CAS No. | 159414-99-0 |
| Molecular Formula | C41H49FN5O8P |
| Molecular Weight | 789.8 |
| PubChem CID | 67090252 |
| InChIKey | CNFKJHKDSRXNFL-UTXREMQHSA-N |
| Purity | 100% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-fluoro RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N4-Acetyl
- Packaging
- 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.3%
- Physical form
- white to off-white powder
- Stability
- powder: 3 years at -20 C
Contexte d’application
- 2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- 5'-DMT + N4-acetyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 67090252
PubChem · CID 67090252
Questions fréquentes
What does this 2'-F amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-acetyl removed at final deprotection. The product provides a 2'-fluoro cytidine phosphoramidite identity.
How does it differ from the 2'-F-dC(Bz) phosphoramidite?
Both are 2'-fluoro cytidine amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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