2'-O-MOE-5-Methyluridine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C43H55N4O10P
- PM
- 818.9
- Pureté
- 100% (HPLC)
2'-O-MOE-5-Methyluridine CE Phosphoramidite is the 2'-O-methoxyethyl 5-methyluridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-5-methyluridine (5-methyluracil base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65036 is identified by CAS 163878-63-5, molecular formula C43H55N4O10P, molecular weight 818.9, PubChem CID 92043625, and InChIKey YFRRKZDUDXHJNC-KZQAAKLLSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonymes
DMT-2'-O-MOE-5Me-rU phosphoramidite; 5'-O-DMT-2'-O-MOE-5-methyluridine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65036 |
| CAS No. | 163878-63-5 |
| Molecular Formula | C43H55N4O10P |
| Molecular Weight | 818.9 |
| PubChem CID | 92043625 |
| InChIKey | YFRRKZDUDXHJNC-KZQAAKLLSA-N |
| Purity | 100% (HPLC) |
Conditions de stockage
-20 C
Caractéristiques techniques
- Monomer class
- 2'-O-MOE RNA CE phosphoramidite
- Nucleobase
- 5-Methyluracil
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- lot expiration 5 September 2026
Contexte d’application
- 2'-MOE RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies and as gapmer flank residues.
- No base protection needed: the 5-methyluracil base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 92043625
PubChem · CID 92043625
Questions fréquentes
Why does this 2'-MOE amidite have no base protecting group?
Its base is 5-methyluracil, which has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-O-MOE-5-methyluridine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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