LNA-G(dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C44H53N8O8P
- PM
- 852.9
- Pureté
- 100% (HPLC)
LNA-G(dmf) CE Phosphoramidite is the locked nucleic acid (LNA) guanosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N2-dimethylformamidine-guanosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65049 is identified by CAS 709641-79-2, molecular formula C44H53N8O8P, molecular weight 852.9, PubChem CID 161667440, and InChIKey ACJXYPZQPQPGNE-KPKUBJDASA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection.
Synonymes
DMT-LNA-G(dmf) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N2-dmf-guanosine 3'-CE phosphoramidite
Identité et spécifications
| Catalog No. | CH65049 |
| CAS No. | 709641-79-2 |
| Molecular Formula | C44H53N8O8P |
| Molecular Weight | 852.9 |
| PubChem CID | 161667440 |
| InChIKey | ACJXYPZQPQPGNE-KPKUBJDASA-N |
| Purity | 100% (HPLC) |
Conditions de stockage
-20 C; dry and inert atmosphere
Caractéristiques techniques
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- N2-dimethylformamidine
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- K.F. <=0.50% w/w
- Physical form
- white, off-white to faint yellow powder, free from visible foreign matter
- Stability
- >=4 years
Contexte d’application
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- 5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 161667440
PubChem · CID 161667440
Questions fréquentes
What residue identity does this LNA amidite provide?
It provides the locked (LNA) guanosine residue identity through its 3'-phosphoramidite coupling group, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection.
How does it differ from the LNA-G(iBu) amidite?
Both are LNA (locked) guanosine phosphoramidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
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