5-ap-2'-dCTP (Aminopropargyl-dCTP)
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C12H19N4O13P3
- PM
- 520.22
- Pureté
- >=98%
5-ap-2'-dCTP is 5-(3-aminopropargyl)-2'-deoxycytidine-5'-triphosphate: a modified dCTP carrying a primary amine on a propargyl (alkyne) linker at the C5 of cytosine. Key identifiers include CAS 115899-39-3, molecular formula C12H19N4O13P3, and molecular weight 520.22 (PubChem CID 11283796).
DNA polymerases incorporate deoxynucleoside triphosphate substrates during template-directed DNA synthesis. This amine-modified dCTP places a primary amine into the DNA; that aliphatic amine then reacts specifically with amine-reactive reporters — such as NHS-ester dyes — so the DNA can be labeled after synthesis.
Synonymes
5-(3-aminopropargyl)-2'-deoxycytidine-5'-triphosphate; 5-propargylamino-dCTP; AP-dCTP
Identité et spécifications
| Catalog No. | CH66013 |
| CAS No. | 115899-39-3 |
| Molecular Formula | C12H19N4O13P3 |
| Molecular Weight | 520.22 |
| PubChem CID | 11283796 |
| InChIKey | LVTQIVFSMGDIPF-IVZWLZJFSA-N |
| Purity | >=98% |
Conditions de stockage
-20 C
Caractéristiques techniques
- Nucleotide class
- dNTP
- Parent nucleotide
- dCTP
- Base modification
- 5-(3-Aminopropargyl)
- Reactive handle
- Primary amine
- Packaging
- 1 mg; 5 mg; 10 mg; 25 mg; 10 µL; 50 µL; 100 µL; 5 x 10 uL; Custom packaging on request
- Solution concentration
- 100-110 mM
- Physical form
- solution in water; slightly yellow
- Stability
- 12 months after delivery; cumulative ambient exposure up to 1 week possible
Contexte d’application
- Amine label handle in DNA: incorporated by DNA polymerase during enzymatic DNA synthesis, placing a primary amine (on a C5 propargyl linker) into the product.
- Post-synthetic dye labeling: the amine reacts with amine-reactive reporters (e.g. NHS-ester dyes) to label the DNA.
- Cytosine analogue: installs the label at cytosine positions during enzymatic DNA synthesis.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 11283796
PubChem · CID 11283796
Questions fréquentes
What is 5-ap-2'-dCTP used for?
It is a 5-aminopropargyl-dCTP: a modified dCTP with a primary amine on a propargyl linker at cytosine. DNA polymerases incorporate it during enzymatic DNA synthesis, placing an amine in the DNA; the amine then reacts with amine-reactive dyes (e.g. NHS esters) to label the product.
Why label with an amine instead of a pre-attached dye?
A small amine linker is incorporated more efficiently by polymerases than a bulky dye. The dye is attached afterward, via the amine, giving efficient incorporation and flexible choice of reporter.
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