7-ap-7-Deaza-2'-dGTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C14H20N5O13P3
- PM
- 559.26
- Pureté
- >98.00%
7-ap-7-Deaza-2'-dGTP is 7-(3-aminopropargyl)-7-deaza-2'-deoxyguanosine-5'-triphosphate: a 7-deazaguanine dGTP analogue (the purine N7 is replaced by C7) carrying a primary amine on a propargyl (alkyne) linker at that C7 position. Key identifiers include CAS 587848-73-5, molecular formula C14H20N5O13P3, and molecular weight 559.26 (PubChem CID 162640848).
DNA polymerases incorporate deoxynucleoside triphosphate substrates during template-directed DNA synthesis. This amine-modified 7-deaza-dGTP places a primary amine into the DNA — presented in the major groove by the 7-deaza scaffold — and that aliphatic amine then reacts specifically with amine-reactive reporters such as NHS-ester dyes, so the DNA can be labeled after synthesis.
Synonymes
7-(3-aminopropargyl)-7-deaza-2'-deoxyguanosine-5'-triphosphate; 7-deaza-7-propargylamino-dGTP
Identité et spécifications
| Catalog No. | CH66017 |
| CAS No. | 587848-73-5 |
| Molecular Formula | C14H20N5O13P3 |
| Molecular Weight | 559.26 |
| PubChem CID | 162640848 |
| InChIKey | VJFQAGRGAURMEX-IVZWLZJFSA-N |
| Purity | >98.00% |
Conditions de stockage
-20 C
Caractéristiques techniques
- Nucleotide class
- dNTP analogue
- Nucleobase
- 7-Deazaguanine
- Base modification
- 7-(3-Aminopropargyl)
- Reactive handle
- Primary amine
- Packaging
- 1 mg; 50 mg; 100 mg; 250 mg; 500 mg; 10 µL; 50 µL; 100 µL; 1 µmol; 10 x 100 uL; 5 x 100 uL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- solid; off-white to slightly yellowish-brown
- Stability
- 12 months after delivery; cumulative ambient exposure up to 1 week possible
Contexte d’application
- Amine label handle at guanine: incorporated by DNA polymerase during enzymatic DNA synthesis, placing a primary amine (on a C7 propargyl linker) at guanine positions.
- 7-deaza scaffold: the purine N7 is replaced by C7, positioning the label in the major groove.
- Post-synthetic dye labeling: the amine reacts with amine-reactive reporters (e.g. NHS-ester dyes) to label the DNA.
Ressources techniques associées
Sources publiques
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Composé PubChem 162640848
PubChem · CID 162640848
Questions fréquentes
What is 7-ap-7-Deaza-2'-dGTP used for?
It is a 7-deaza-dGTP analogue with a primary amine on a C7 propargyl linker. DNA polymerases incorporate it during enzymatic DNA synthesis at guanine positions, placing an amine in the DNA; the amine then reacts with amine-reactive dyes (e.g. NHS esters) to label the product.
What does '7-deaza' mean?
In a 7-deaza purine, the nitrogen at position 7 of the purine ring is replaced by a carbon. This provides an attachment point (C7) for the label in the major groove and is a common scaffold for labeled purine nucleotides.
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