3'-O-NH2-2'-dGTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Résumé du produit
Aperçu rapide
- Famille de produits
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Pureté
- 99.0%
3'-O-NH2-2'-dGTP is a 3'-O-amino modified dGTP reversible terminator. The 3'-hydroxyl is capped with a reversible blocking group so that polymerase extension stops after a single incorporation cycle. Key identifiers are CHEMOS CH66028, CAS 1220515-98-9, molecular formula C10H17N6O13P3, and formula-derived molecular weight 522.20.
3'-O-modified nucleotides are used in cyclic reversible-terminator sequencing-by-synthesis workflows: DNA polymerase incorporates the nucleotide, extension pauses after one base, and cap removal regenerates the 3'-OH so synthesis can continue.
Synonymes
3'-O-amino-dGTP; 3'-O-(aminooxy)-2'-deoxyguanosine-5'-triphosphate
Identité et spécifications
| Catalog No. | CH66028 |
| Product Name | 3'-O-NH2-2'-dGTP |
| CAS No. | 1220515-98-9 |
| Molecular Formula | C10H17N6O13P3 |
| Molecular Weight | 522.20 |
| Product Type | 3'-O-amino reversible-terminator dGTP |
| Base Added | Guanine |
| Purity | 99.0% |
Conditions de stockage
material in solvent at -80 C
Caractéristiques techniques
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dGTP
- 3'-O blocking group
- Amino
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 100 µL; 500 µL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- liquid; colorless to light yellow
- Stability
- 1 year in solvent at -80 C
Contexte d’application
- Reversible terminator nucleotide: used as the guanine member of a 3'-O-blocked dNTP set.
- Sequencing-by-synthesis context: incorporation pauses extension for one cycle before cap removal allows synthesis to continue.
- Modified nucleotide cataloging: relevant to oligonucleotide synthesis, sequencing reagent, and modified dNTP product organization.
Ressources techniques associées
Sources publiques
- 3'-ONH2-dGTP sodium solution, catalog HY-158713
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
Questions fréquentes
What is 3'-O-NH2-2'-dGTP?
3'-O-NH2-2'-dGTP is a 3'-O-amino modified dGTP used as a reversible-terminator nucleotide in sequencing-by-synthesis research workflows.
How does a reversible terminator work?
DNA polymerase incorporates the nucleotide, the 3'-O blocking group pauses further extension, and removal of the cap regenerates the 3'-OH so synthesis can continue.
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