LNA-5-Me-U CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C41H49N4O9P
- 分子量
- 772.8
- 纯度
- 99.3% (NMR)
LNA-5-Me-U CE Phosphoramidite is the locked nucleic acid (LNA) 5-methyluridine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) 5-methyluridine (5-methyluracil base) with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65048 is identified by CAS 206055-75-6, molecular formula C41H49N4O9P, molecular weight 772.8, PubChem CID 137628654, and InChIKey STPXOEPMLLHUDQ-DMMPMGKKSA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required.
同义词
DMT-LNA-5Me-U phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-5-methyluridine 3'-CE phosphoramidite
身份与规格
| Catalog No. | CH65048 |
| CAS No. | 206055-75-6 |
| Molecular Formula | C41H49N4O9P |
| Molecular Weight | 772.8 |
| PubChem CID | 137628654 |
| InChIKey | STPXOEPMLLHUDQ-DMMPMGKKSA-N |
| Purity | 99.3% (NMR) |
储存条件
-20 C
技术属性
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- 5-Methyluracil
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- None required
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
应用背景
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- No base protection needed: the 5-methyluracil base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 137628654
PubChem · CID 137628654
常见问题
Why does this LNA amidite have no base protecting group?
Its base is 5-methyluracil, which has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
What is LNA?
LNA (locked nucleic acid) contains a rigid 2'-O,4'-C-methylene bridge. In this product, that locked sugar is part of a 5-methyluridine phosphoramidite building block for oligonucleotide synthesis.
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