Oligonucleotide Synthesis, RNA Raw Materials & Modification

Thymidine (2'-Deoxythymidine)

1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione

产品编号CH64004CAS号50-89-5纯度99.9% (HPLC)

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
化学名称: 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
分子式: C10H14N2O5
分子量: 242.23
纯度: 99.9% (HPLC)

Thymidine, also written 2'-deoxythymidine (dT), is the thymine DNA nucleoside formed from thymine and 2-deoxy-β-D-ribose. CH64004 is identified by CAS 50-89-5, molecular formula C10H14N2O5, molecular weight 242.23, and PubChem CID 5789.

The source label "2'-deoxythymine" should be read as thymidine / 2'-deoxythymidine, not as the free base thymine. Thymidine is used in thymidine-kinase phosphorylation research and is the parent nucleoside behind tritiated-thymidine DNA-synthesis assays.

同义词

Thymidine; 2'-deoxythymidine; dT; deoxythymidine; thymine 2'-deoxyriboside

身份与规格

Catalog No.	CH64004
CAS No.	50-89-5
Molecular Formula	C10H14N2O5
Molecular Weight	242.23
PubChem CID	5789
InChIKey	IQFYYKKMVGJFEH-XLPZGREQSA-N
Purity	99.9% (HPLC)

技术属性

Nucleobase: Thymine (5-methyluracil)
Sugar: 2'-Deoxyribose
Nucleoside class: Pyrimidine 2'-deoxyribonucleoside
Packaging: Custom packaging on request
Water content: <=0.5% KF
Physical form: White to almost white crystalline powder
Stability: Released 2024-05-13; retest May 2029

应用背景

Thymidine-kinase research: literature reports thymidine as a substrate of thymidine kinase in phosphorylation studies.
DNA-synthesis assays: tritiated thymidine ([3H]thymidine) is a classic DNA-synthesis marker, with thymidine as the parent nucleoside.
Thymine DNA nucleoside reference: CH64004 provides the dT identity for nucleoside and oligonucleotide chemistry comparisons.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 5789
PubChem · CID 5789

常见问题

Is CH64004 thymidine or the base thymine?

It is thymidine, the nucleoside (CAS 50-89-5) — thymine joined to 2-deoxyribose. The brochure label "2'-deoxythymine" is imprecise; the free base thymine is a separate compound.

Why is thymidine used in DNA-synthesis assays?

Tritiated thymidine ([3H]thymidine) is incorporated into newly synthesized DNA, making it a classic reference marker for DNA synthesis; thymidine is the parent nucleoside for these assays.