5'-O-DMT-thymidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C31H32N2O7
- 分子量
- 544.60
- 纯度
- >=98.5% (HPLC)
5'-O-DMT-thymidine is thymidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group. CH64102 is identified by CAS 40615-39-2, molecular formula C31H32N2O7, molecular weight 544.60, PubChem CID 162419, and InChIKey UBTJZUKVKGZHAD-UPRLRBBYSA-N.
It is a 5'-protected precursor for DNA phosphoramidite/oligonucleotide synthesis: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle. Because thymine has no exocyclic amine, no base protecting group is required.
同义词
5'-O-DMT-thymidine; 5'-O-(4,4'-dimethoxytrityl)thymidine; DMT-dT
身份与规格
| Catalog No. | CH64102 |
| CAS No. | 40615-39-2 |
| Molecular Formula | C31H32N2O7 |
| Molecular Weight | 544.60 |
| PubChem CID | 162419 |
| InChIKey | UBTJZUKVKGZHAD-UPRLRBBYSA-N |
| Purity | >=98.5% (HPLC) |
储存条件
2-8 C
技术属性
- Nucleobase
- Thymine (5-methyluracil)
- Sugar
- 2'-Deoxyribose
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 5 g; 25 g; 100 g; 250 g; 500 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder to crystalline powder
- Stability
- >=4 years
应用背景
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each oligonucleotide chain-elongation cycle.
- No base protection needed: thymine has no exocyclic amine, so this building block requires no base protecting group.
- Phosphoramidite precursor context: a 5'-protected thymidine precursor toward DNA phosphoramidite building blocks.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 162419
PubChem · CID 162419
常见问题
Why does 5'-O-DMT-thymidine have no base protecting group?
Thymine has no reactive exocyclic amine, so unlike A, C, and G it needs no base protection. Only the 5'-hydroxyl is protected with the acid-labile DMT group, which is removed at the start of each oligonucleotide chain-elongation cycle.
How does it differ from thymidine?
It carries a 5'-O-(4,4'-dimethoxytrityl) group on the 5'-hydroxyl, whereas thymidine is unprotected. Thymidine is cataloged separately.
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