Oligonucleotide Synthesis, RNA Raw Materials & Modification

5'-O-DMT-N4-benzoyl-2'-O-methylcytidine

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH64117CAS号110764-74-4纯度99.52%

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C38H37N3O8
分子量: 663.7
纯度: 99.52%

5'-O-DMT-N4-benzoyl-2'-O-methylcytidine is 2'-O-methylcytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-benzoyl base-protecting group. CH64117 is identified by CAS 110764-74-4, molecular formula C38H37N3O8, molecular weight 663.7, PubChem CID 13872214, and InChIKey WXJKGOQQYUVNQW-YDXJMRNDSA-N.

It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N4-benzoyl group masks the cytosine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

同义词

5'-O-DMT-N4-benzoyl-2'-O-methylcytidine; 5'-O-DMT-2'-OMe-rC(Bz); DMT-2'-OMe-rC(Bz)

身份与规格

Catalog No.	CH64117
CAS No.	110764-74-4
Molecular Formula	C38H37N3O8
Molecular Weight	663.7
PubChem CID	13872214
InChIKey	WXJKGOQQYUVNQW-YDXJMRNDSA-N
Purity	99.52%

储存条件

2-8 C

技术属性

Nucleobase: Cytosine
5'-O protection: DMT (dimethoxytrityl)
Sugar modification: 2'-O-Methyl
Base protection: N4-Benzoyl
Packaging: 5 mg; 500 mg; 1 g; 5 g; 10 g; 50 g; 100 g; 250 g; 500 g; Custom packaging on request
Water content: <=1.0%
Physical form: white to off-white powder
Stability: 3 years at -20 C

应用背景

5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
2'-OMe modification context: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
N4-benzoyl base protection: the benzoyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 13872214
PubChem · CID 13872214

常见问题

How does it differ from N4-benzoyl-2'-O-methylcytidine?

Both carry an N4-benzoyl-protected 2'-O-methylcytidine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.