Oligonucleotide Synthesis, RNA Raw Materials & Modification

5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH64118CAS号114745-26-5纯度99%

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C36H39N5O8
分子量: 669.7
纯度: 99%

5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine is 2'-O-methylguanosine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N2-isobutyryl base-protecting group. CH64118 is identified by CAS 114745-26-5, molecular formula C36H39N5O8, molecular weight 669.7, PubChem CID 135742507, and InChIKey ISQLJOGRNUQHJX-WIFIACMTSA-N.

It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N2-isobutyryl group masks the guanine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

同义词

5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine; 5'-O-DMT-2'-OMe-rG(iBu); DMT-2'-OMe-rG(iBu)

身份与规格

Catalog No.	CH64118
CAS No.	114745-26-5
Molecular Formula	C36H39N5O8
Molecular Weight	669.7
PubChem CID	135742507
InChIKey	ISQLJOGRNUQHJX-WIFIACMTSA-N
Purity	99%

储存条件

-20 C

技术属性

Nucleobase: Guanine
5'-O protection: DMT (dimethoxytrityl)
Sugar modification: 2'-O-Methyl
Base protection: N2-Isobutyryl
Grade: N (Normal)
Packaging: 25 mg; 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; Custom packaging on request
Water content: <=1.0%
Physical form: white to off-white powder
Stability: 3 years at -20 C; 2 years at 4 C

应用背景

5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
2'-OMe modification context: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 135742507
PubChem · CID 135742507

常见问题

How does it differ from N2-isobutyryl-2'-O-methylguanosine?

Both carry an N2-isobutyryl-protected 2'-O-methylguanosine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.