Oligonucleotide Synthesis, RNA Raw Materials & Modification

5'-O-DMT-N4-acetyl-2'-O-methylcytidine

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH64121CAS号199593-08-3纯度>=99%

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C33H35N3O8
分子量: 601.6
纯度: >=99%

5'-O-DMT-N4-acetyl-2'-O-methylcytidine is 2'-O-methylcytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-acetyl base-protecting group. CH64121 is identified by CAS 199593-08-3, molecular formula C33H35N3O8, molecular weight 601.6, PubChem CID 70700566, and InChIKey FINUHOJILDNELQ-PMFUCWTESA-N.

It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N4-acetyl group masks the cytosine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

同义词

5'-O-DMT-N4-acetyl-2'-O-methylcytidine; 5'-O-DMT-2'-OMe-rC(Ac); DMT-2'-OMe-rC(Ac)

身份与规格

Catalog No.	CH64121
CAS No.	199593-08-3
Molecular Formula	C33H35N3O8
Molecular Weight	601.6
PubChem CID	70700566
InChIKey	FINUHOJILDNELQ-PMFUCWTESA-N
Purity	>=99%

储存条件

2-8 C

技术属性

Nucleobase: Cytosine
5'-O protection: DMT (dimethoxytrityl)
Sugar modification: 2'-O-Methyl
Base protection: N4-Acetyl
Packaging: 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; 1 kg; Custom packaging on request
Water content: <=1.0%
Physical form: white to off-white powder
Stability: Powder: 3 years at -20 C; 2 years at 4 C; in solvent: 6 months at -80 C; 1 month at -20 C

应用背景

5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 70700566
PubChem · CID 70700566

常见问题

How does it differ from 5'-O-DMT-N4-benzoyl-2'-O-methylcytidine?

Both are 5'-O-DMT-protected 2'-O-methylcytidines with a protected cytosine N4-amine, but this one uses an acetyl group rather than benzoyl. The benzoyl form is cataloged separately.