Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-Deoxycytidine (Ac) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65005CAS号154110-40-4纯度100% (NMR)

暂无产品图片

产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C41H50N5O8P
分子量: 771.8
纯度: 100% (NMR)

2'-Deoxycytidine (Ac) CE Phosphoramidite is the fully protected 2'-deoxycytidine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-acetyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65005 is identified by CAS 154110-40-4, molecular formula C41H50N5O8P, molecular weight 771.8, PubChem CID 70700205, and InChIKey MECWEBCHRKVTNV-RLWDVSNXSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the N4-acetyl base group is removed at final deprotection.

同义词

DMT-dC(Ac) phosphoramidite; N4-acetyl-5'-O-DMT-2'-deoxycytidine 3'-CE phosphoramidite

身份与规格

Catalog No.	CH65005
CAS No.	154110-40-4
Molecular Formula	C41H50N5O8P
Molecular Weight	771.8
PubChem CID	70700205
InChIKey	MECWEBCHRKVTNV-RLWDVSNXSA-N
Purity	100% (NMR)

储存条件

-20 C

技术属性

Monomer class: DNA CE phosphoramidite
Nucleobase: Cytosine
5'-O protection: DMT (dimethoxytrityl)
Base protection: N4-Acetyl
Grade: Standard; TheraPure
Packaging: 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; 500 g; 1 kg; 6 x 10 g; 6 x 20 g; 6 x 5 g; Custom packaging on request
Water content: <=0.3% w/w
Physical form: white to yellow powder
Stability: Specification print date 2026-01-01

应用背景

DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 70700205
PubChem · CID 70700205

常见问题

What makes this a phosphoramidite building block?

It carries a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group — the reactive coupling group used in automated solid-phase DNA synthesis. It also has an acid-labile 5'-O-DMT group (removed each cycle) and an N4-acetyl base protection (removed at final deprotection).

How does it differ from 2'-deoxycytidine (Bz) phosphoramidite?

Both are 5'-O-DMT 2'-deoxycytidine phosphoramidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.