2'-Deoxyinosine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C40H47N6O7P
- 分子量
- 754.8
- 纯度
- 99% (NMR)
2'-Deoxyinosine CE Phosphoramidite is the 2'-deoxyinosine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyinosine (hypoxanthine base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65008 is identified by CAS 141684-35-7, molecular formula C40H47N6O7P, molecular weight 754.8, PubChem CID 135741319, and InChIKey ZKGZROFWRPJVEB-XZYUMQGLSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, and the acid-labile 5'-DMT is removed at the start of each cycle. Because the hypoxanthine base has no exocyclic amine, no base protecting group is required.
同义词
DMT-dI phosphoramidite; 5'-O-DMT-2'-deoxyinosine 3'-CE phosphoramidite
身份与规格
| Catalog No. | CH65008 |
| CAS No. | 141684-35-7 |
| Molecular Formula | C40H47N6O7P |
| Molecular Weight | 754.8 |
| PubChem CID | 135741319 |
| InChIKey | ZKGZROFWRPJVEB-XZYUMQGLSA-N |
| Purity | 99% (NMR) |
储存条件
at or below -20 C
技术属性
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Hypoxanthine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- Unprotected
- Grade
- Standard
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 100 µmol; Custom packaging on request
- Water content
- K.F. <=0.20% w/w
- Coupling efficiency
- spec >=98.0% and observed 98.0%
- Physical form
- powder
- Stability
- Manufactured 2021-12-30; retest 2025-07-02; expired 2025-12-29
应用背景
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- No base protection needed: the hypoxanthine base has no exocyclic amine, so this monomer requires no base protecting group.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 135741319
PubChem · CID 135741319
常见问题
Why does the 2'-deoxyinosine phosphoramidite have no base protecting group?
The hypoxanthine base of 2'-deoxyinosine has no reactive exocyclic amine, so it needs no base protection. The monomer carries only the acid-labile 5'-O-DMT group (removed each cycle) and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-deoxyinosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The 5'-O-DMT-2'-deoxyinosine precursor is cataloged separately.
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