Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-Cytidine (Ac) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65018CAS号199593-09-4纯度>=99% (NMR)

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C42H52N5O9P
分子量: 801.9
纯度: >=99% (NMR)

2'-OMe-Cytidine (Ac) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-acetyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65018 is identified by CAS 199593-09-4, molecular formula C42H52N5O9P, molecular weight 801.9, PubChem CID 66764086, and InChIKey WNWUMIPFLOKTEZ-UAQIPLLRSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

同义词

DMT-2'-OMe-rC(Ac) phosphoramidite; 5'-O-DMT-N4-acetyl-2'-O-methylcytidine 3'-CE phosphoramidite

身份与规格

Catalog No.	CH65018
CAS No.	199593-09-4
Molecular Formula	C42H52N5O9P
Molecular Weight	801.9
PubChem CID	66764086
InChIKey	WNWUMIPFLOKTEZ-UAQIPLLRSA-N
Purity	>=99% (NMR)

储存条件

-20 C; under inert atmosphere

技术属性

Monomer class: 2'-O-methyl RNA CE phosphoramidite
Nucleobase: Cytosine
Sugar modification: 2'-O-Methyl
Base protection: N4-Acetyl
Grade: Standard; TheraPure
Packaging: 250 mg; 500 mg; 1 g; 5 g; 10 g; 100 g; Custom packaging on request
Water content: <=0.3 wt.% by Karl Fischer
Physical form: white to off-white powder
Stability: >=4 years

应用背景

2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
5'-DMT + N4-acetyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 66764086
PubChem · CID 66764086

常见问题

How does it differ from the 2'-OMe-C(Bz) phosphoramidite?

Both are 2'-O-methyl cytidine amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.