Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-Cytidine (Bz) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65023CAS号110764-78-8纯度>99.00%

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C47H54N5O9P
分子量: 863.9
纯度: >99.00%

2'-OMe-Cytidine (Bz) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65023 is identified by CAS 110764-78-8, molecular formula C47H54N5O9P, molecular weight 863.9, PubChem CID 13872225, and InChIKey JFFSFQRVIPPCBC-VKBHKTMGSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

同义词

DMT-2'-OMe-rC(Bz) phosphoramidite; 5'-O-DMT-N4-benzoyl-2'-O-methylcytidine 3'-CE phosphoramidite

身份与规格

Catalog No.	CH65023
CAS No.	110764-78-8
Molecular Formula	C47H54N5O9P
Molecular Weight	863.9
PubChem CID	13872225
InChIKey	JFFSFQRVIPPCBC-VKBHKTMGSA-N
Purity	>99.00%

储存条件

-20 C

技术属性

Monomer class: 2'-O-methyl RNA CE phosphoramidite
Nucleobase: Cytosine
Sugar modification: 2'-O-Methyl
Base protection: N4-Benzoyl
Grade: N (Normal)
Packaging: 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 25 g; 100 g; Custom packaging on request
Physical form: white powder
Stability: >=4 years

应用背景

2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
5'-DMT + N4-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 13872225
PubChem · CID 13872225

常见问题

What does this 2'-OMe amidite do in RNA synthesis?

Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-benzoyl removed at final deprotection. The product provides a 2'-O-methylcytidine phosphoramidite identity.

How does it differ from the 2'-OMe-C(Ac) phosphoramidite?

Both are 2'-O-methyl cytidine amidites, but this one uses an N4-benzoyl base protection rather than acetyl. The acetyl amidite is cataloged separately.