Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-F-Cytidine (Ac) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65030CAS号159414-99-0纯度100% (HPLC)

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C41H49FN5O8P
分子量: 789.8
纯度: 100% (HPLC)

2'-F-Cytidine (Ac) CE Phosphoramidite is the 2'-fluoro cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N4-acetyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65030 is identified by CAS 159414-99-0, molecular formula C41H49FN5O8P, molecular weight 789.8, PubChem CID 67090252, and InChIKey CNFKJHKDSRXNFL-UTXREMQHSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.

同义词

DMT-2'-F-rC(Ac) phosphoramidite; 5'-O-DMT-2'-fluoro-N4-acetyl-2'-deoxycytidine 3'-CE phosphoramidite

身份与规格

Catalog No.	CH65030
CAS No.	159414-99-0
Molecular Formula	C41H49FN5O8P
Molecular Weight	789.8
PubChem CID	67090252
InChIKey	CNFKJHKDSRXNFL-UTXREMQHSA-N
Purity	100% (HPLC)

储存条件

-20 C

技术属性

Monomer class: 2'-fluoro RNA CE phosphoramidite
Nucleobase: Cytosine
Sugar modification: 2'-Fluoro-2'-deoxy
Base protection: N4-Acetyl
Packaging: 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
Water content: <=0.3%
Physical form: white to off-white powder
Stability: powder: 3 years at -20 C

应用背景

2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
5'-DMT + N4-acetyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 67090252
PubChem · CID 67090252

常见问题

What does this 2'-F amidite do in RNA synthesis?

Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-acetyl removed at final deprotection. The product provides a 2'-fluoro cytidine phosphoramidite identity.

How does it differ from the 2'-F-dC(Bz) phosphoramidite?

Both are 2'-fluoro cytidine amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.