2'-O-MOE-5-Methyluridine CE Phosphoramidite

2'-O-MOE-5-Methyluridine CE Phosphoramidite is the 2'-O-methoxyethyl 5-methyluridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-5-methyluridine (5-methyluracil base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65036 is identified by CAS 163878-63-5, molecular formula C43H55N4O10P, molecular weight 818.9, PubChem CID 92043625, and InChIKey YFRRKZDUDXHJNC-KZQAAKLLSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.