Oligonucleotide Synthesis, RNA Raw Materials & Modification

LNA-G(dmf) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65049CAS号709641-79-2纯度100% (HPLC)

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C44H53N8O8P
分子量: 852.9
纯度: 100% (HPLC)

LNA-G(dmf) CE Phosphoramidite is the locked nucleic acid (LNA) guanosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N2-dimethylformamidine-guanosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65049 is identified by CAS 709641-79-2, molecular formula C44H53N8O8P, molecular weight 852.9, PubChem CID 161667440, and InChIKey ACJXYPZQPQPGNE-KPKUBJDASA-N.

The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection.

同义词

DMT-LNA-G(dmf) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N2-dmf-guanosine 3'-CE phosphoramidite

身份与规格

Catalog No.	CH65049
CAS No.	709641-79-2
Molecular Formula	C44H53N8O8P
Molecular Weight	852.9
PubChem CID	161667440
InChIKey	ACJXYPZQPQPGNE-KPKUBJDASA-N
Purity	100% (HPLC)

储存条件

-20 C; dry and inert atmosphere

技术属性

Monomer class: LNA CE phosphoramidite
Nucleobase: Guanine
Sugar constraint: 2'-O,4'-C-methylene bridge
Base protection: N2-dimethylformamidine
Grade: N (Normal)
Packaging: 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
Water content: K.F. <=0.50% w/w
Physical form: white, off-white to faint yellow powder, free from visible foreign matter
Stability: >=4 years

应用背景

LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 161667440
PubChem · CID 161667440

常见问题

What residue identity does this LNA amidite provide?

It provides the locked (LNA) guanosine residue identity through its 3'-phosphoramidite coupling group, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection.

How does it differ from the LNA-G(iBu) amidite?

Both are LNA (locked) guanosine phosphoramidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.