7-ap-7-Deaza-ddATP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C14H20N5O11P3
- 分子量
- 527.26
- 纯度
- >=95% (HPLC)
7-ap-7-Deaza-ddATP is 7-(3-aminopropargyl)-7-deaza-2',3'-dideoxyadenosine-5'-triphosphate: a 7-deaza (purine N7 replaced by C7) 2',3'-dideoxy ATP chain terminator carrying a primary amine on a propargyl (alkyne) linker at C7. Key identifiers include CAS 114748-69-5, molecular formula C14H20N5O11P3, and molecular weight 527.26 (PubChem CID 11249496).
2',3'-dideoxynucleoside triphosphates act as specific chain-terminating inhibitors of DNA polymerase — the basis of Sanger dideoxy sequencing: once one is incorporated, the missing 3'-OH stops further extension. The C7 primary amine is a labelling handle for attaching a dye, presented in the major groove by the 7-deaza scaffold, so this reagent serves as a building block for labeled dye-terminators.
同义词
7-(3-aminopropargyl)-7-deaza-2',3'-dideoxyadenosine-5'-triphosphate; amino-7-deaza-ddATP
身份与规格
| Catalog No. | CH66020 |
| CAS No. | 114748-69-5 |
| Molecular Formula | C14H20N5O11P3 |
| Molecular Weight | 527.26 |
| PubChem CID | 11249496 |
| InChIKey | LQOGZFINDBWPBN-WDEREUQCSA-N |
| Purity | >=95% (HPLC) |
储存条件
-20 C
技术属性
- Nucleotide class
- Chain-terminating ddNTP
- Nucleobase
- 7-Deazaadenine
- Base modification
- 7-(3-Aminopropargyl)
- Reactive handle
- Primary amine
- Packaging
- 1 mg; 50 mg; 100 mg; 250 mg; 500 mg; Custom packaging on request
- Physical form
- solid; white to off-white
- Stability
- 12 months after delivery; cumulative ambient exposure up to 1 week possible
应用背景
- Chain terminator: a 7-deaza 2',3'-dideoxy-ATP that stops DNA-polymerase extension when incorporated (the basis of Sanger sequencing).
- Amine label handle in the major groove: the C7 aminopropargyl group (on the 7-deaza scaffold) lets a dye be attached, giving a labeled terminator.
- Dye-terminator building block: used to prepare fluorescent ddNTP terminators for sequencing.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 11249496
PubChem · CID 11249496
常见问题
What is 7-ap-7-Deaza-ddATP used for?
It is a 7-deaza 2',3'-dideoxy-ATP chain terminator with a C7 aminopropargyl amine handle. As a dideoxy nucleotide it stops DNA-polymerase extension (the basis of Sanger sequencing); the amine allows a dye to be attached, making it a building block for labeled dye-terminators.
Why the 7-deaza modification on a dideoxy terminator?
Replacing the purine N7 with C7 provides an attachment point in the major groove for the label and avoids a hydrogen-bonding nitrogen there. Combined with the missing 3'-OH (which terminates synthesis), it gives a well-behaved labeled purine terminator.
相似产品
需要协助选择物料？
CHEMOS 可协助评估产品匹配、目标结构、路线问题、分析要求和项目供应需求。