3'-O-allyl-2'-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C13H21N2O14P3
- 分子量
- 522.23
- 纯度
- >=98%
3'-O-allyl-2'-dTTP is 3'-O-allyl-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with an allyl (prop-2-en-1-yl) group. Key identifiers include CAS 917766-47-3, molecular formula C13H21N2O14P3, and molecular weight 522.23 (PubChem CID 77134385).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-allyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
同义词
3'-O-allyl-dTTP; 3'-O-(prop-2-en-1-yl)-thymidine-5'-triphosphate
身份与规格
| Catalog No. | CH66025 |
| CAS No. | 917766-47-3 |
| Molecular Formula | C13H21N2O14P3 |
| Molecular Weight | 522.23 |
| PubChem CID | 77134385 |
| InChIKey | JGJOVFWVTMUSDJ-HBNTYKKESA-N |
| Purity | >=98% |
技术属性
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Allyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- Custom packaging on request
应用背景
- Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-allyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Thymine building block: supplies the thymine reversible terminator in a four-nucleotide SBS set.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 77134385
PubChem · CID 77134385
常见问题
What is a 3'-O-allyl reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with an allyl group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the allyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the thymine (T) reversible terminator — 3'-O-allyl-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-allyl terminators it forms a four-nucleotide set for sequencing-by-synthesis.
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