3'-O-NH2-2'-dCTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C9H17N4O13P3
- 分子量
- 482.17
- 纯度
- 99.0%
3'-O-NH2-2'-dCTP is 3'-O-amino-2'-deoxycytidine-5'-triphosphate: a reversible-terminator dCTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. Key identifiers include CAS 1220515-75-2, molecular formula C9H17N4O13P3, and molecular weight 482.17 (PubChem CID 87229304).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-amino cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
同义词
3'-O-amino-dCTP; 3'-O-(aminooxy)-2'-deoxycytidine-5'-triphosphate
身份与规格
| Catalog No. | CH66027 |
| CAS No. | 1220515-75-2 |
| Molecular Formula | C9H17N4O13P3 |
| Molecular Weight | 482.17 |
| PubChem CID | 87229304 |
| InChIKey | VECVOKKASOJPEY-SHYZEUOFSA-N |
| Purity | 99.0% |
储存条件
material in solvent at -80 C
技术属性
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dCTP
- 3'-O blocking group
- Amino
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 1 mL; 5 mL; 10 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- liquid; colorless to light yellow
- Stability
- 1 year in solvent at -80 C
应用背景
- Reversible terminator: DNA polymerase incorporates it, adding one cytosine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-amino cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Cytosine building block: supplies the cytosine reversible terminator in a four-nucleotide SBS set.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 87229304
PubChem · CID 87229304
常见问题
What is a 3'-O-amino reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the 3'-O-amino cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the cytosine (C) reversible terminator — 3'-O-amino-2'-deoxycytidine-5'-triphosphate.
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