3'-O-cyanoethenyl-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 纯度
- >=98%
3'-O-cyanoethenyl-dTTP is a 3'-O-cyanoethenyl modified dTTP reversible terminator. The 3'-hydroxyl is capped with a reversible blocking group so that polymerase extension stops after a single incorporation cycle. Key identifiers for this catalog entry are CHEMOS CH66035 and CAS 2142998-82-9.
3'-O-modified nucleotides are used in cyclic reversible-terminator sequencing-by-synthesis workflows: DNA polymerase incorporates the nucleotide, extension pauses after one base, and cap removal regenerates the 3'-OH so synthesis can continue. This page keeps the identity boundary to the supplier catalog name, CAS number, and literature-supported reversible-terminator nucleotide context.
同义词
3'-O-cyanoethenyl-dTTP; 3'-O-(2-cyanovinyl)-thymidine-5'-triphosphate
身份与规格
| Catalog No. | CH66035 |
| Product Name | 3'-O-cyanoethenyl-dTTP |
| CAS No. | 2142998-82-9 |
| Product Type | 3'-O-cyanoethenyl reversible-terminator dTTP |
| Base Added | Thymine |
| Purity | >=98% |
技术属性
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Cyanoethenyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- Custom packaging on request
应用背景
- Reversible terminator nucleotide: used as the thymine member of a 3'-O-blocked dNTP set.
- Sequencing-by-synthesis context: incorporation pauses extension for one cycle before cap removal allows synthesis to continue.
- Modified nucleotide cataloging: relevant to oligonucleotide synthesis, sequencing reagent, and modified dNTP product organization.
相关技术资源
公开来源
- 3'-O-cyanoethenyl-dTTP Na3 | CAS 2142998-82-9
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
常见问题
What is 3'-O-cyanoethenyl-dTTP?
3'-O-cyanoethenyl-dTTP is a 3'-O-cyanoethenyl modified dTTP used as a reversible-terminator nucleotide in sequencing-by-synthesis research workflows.
How does a reversible terminator work?
DNA polymerase incorporates the nucleotide, the 3'-O blocking group pauses further extension, and removal of the cap regenerates the 3'-OH so synthesis can continue.
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