Oligonucleotide Synthesis, RNA Raw Materials & Modification

Spacer-C6 Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

产品编号CH65097CAS号158041-82-8纯度>=98%

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产品概述

快速信息

产品系列: Oligonucleotide Synthesis, RNA Raw Materials & Modification
分子式: C36H49N2O5P
分子量: 620.8
纯度: >=98%

Spacer-C6 Phosphoramidite is a non-nucleotide (abasic) spacer building block: a six-carbon (hexyl) spacer carrying a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 158041-82-8, molecular formula C36H49N2O5P, molecular weight 620.8, and PubChem CID 117768145.

Coupling inserts a non-nucleotide six-carbon spacer into the oligonucleotide backbone in place of a nucleotide — non-nucleotide chains can be incorporated into oligonucleotides this way (for example, to join segments or introduce a defined gap). The spacer couples through its 3'-phosphoramidite, and its 5'-DMT is removed each cycle so chain assembly continues.

同义词

DMT-O-C6-spacer 3'-CE phosphoramidite; 6-(DMTO)hexyl non-nucleotide spacer amidite

身份与规格

Catalog No.	CH65097
CAS No.	158041-82-8
Molecular Formula	C36H49N2O5P
Molecular Weight	620.8
PubChem CID	117768145
InChIKey	AEDFSPMNECTXJM-UHFFFAOYSA-N
Purity	>=98%

储存条件

-20 C

技术属性

Reagent class: Non-nucleotide spacer phosphoramidite
Spacer type: Alkyl
Spacer length: 6 carbons
End protection: 5'-O-DMT
Packaging: 250 mg; 500 mg; 1 g; 5 g; 10 g; Custom packaging on request
Water content: <=0.5%
Physical form: colourless transparent oily substance

应用背景

Non-nucleotide spacer insertion: introduces a six-carbon abasic spacer into the oligonucleotide backbone in place of a nucleotide.
Chain-compatible: couples through a 3'-phosphoramidite and carries a 5'-DMT that is removed each cycle, so synthesis can continue past the spacer.
Backbone linker: non-nucleotide chains can be incorporated into oligonucleotides to join segments or introduce a defined gap.

公开来源

Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
PubChem 化合物 117768145
PubChem · CID 117768145

常见问题

What is a spacer phosphoramidite?

It is a non-nucleotide (abasic) building block that inserts a spacer unit into the oligonucleotide backbone in place of a nucleotide. It couples through a 3'-phosphoramidite and carries a 5'-DMT (removed each cycle) so chain assembly continues normally.

What does the 'C6' mean?

C6 denotes the length of the spacer — a six-carbon (hexyl) chain. Shorter (C2, C3) and longer (C12) variants and ethylene-glycol spacers give correspondingly different spacings.