Chemical Phosphorylation Reagent II
Oligonucleotide Synthesis, RNA Raw Materials & Modification
产品概述
快速信息
- 产品系列
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 分子式
- C39H51N2O9P
- 分子量
- 722.8
- 纯度
- 98% (HPLC)
Chemical Phosphorylation Reagent II is a non-nucleoside building block for introducing a phosphate group on an oligonucleotide during synthesis: a phosphoramidite carrying a protected phosphate (with a 5'-O-(4,4'-dimethoxytrityl) handle) plus a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 171285-25-9, molecular formula C39H51N2O9P, molecular weight 722.8, and PubChem CID 10283713.
Like the first Chemical Phosphorylation Reagent, it couples at the oligonucleotide terminus like any amidite; after the phosphate protecting group is removed during deprotection, it leaves a phosphate monoester — for example a 5'-phosphate — chemically, without an enzymatic step. The "II" name distinguishes this no-sulfur protected-phosphate structure from CH65101.
同义词
CPR II; Chemical Phosphorylation Reagent II; 5'-phosphate-ON reagent
身份与规格
| Catalog No. | CH65102 |
| CAS No. | 171285-25-9 |
| Molecular Formula | C39H51N2O9P |
| Molecular Weight | 722.8 |
| PubChem CID | 10283713 |
| InChIKey | IEKIULVSVQLVMS-UHFFFAOYSA-N |
| Purity | 98% (HPLC) |
储存条件
-20 C; dark; desiccated
技术属性
- Reagent class
- Non-nucleoside phosphorylation phosphoramidite
- Installation site
- Oligonucleotide terminus
- Installed group
- Phosphate monoester after deprotection
- Structure distinction
- No-sulfur protected-phosphate structure
- Packaging
- 10 mg; 100 mg; 250 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 10 kg; 50 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colorless semisolid
- Stability
- Product: 12 months after receipt; diluted reagent: <=24 hours under anhydrous conditions; transport: up to 3 weeks at room temperature
应用背景
- Chemical Phosphorylation Reagent II reference: use this chemical phosphorylation reagent record for oligonucleotide phosphorylation reagent catalog work.
- Identifier cross-check: compare CAS 171285-25-9, PubChem CID 10283713, formula C39H51N2O9P, MW 722.8, and the listed InChIKey for catalog identity matching.
- Inquiry preparation: include catalog number CH65102, the product name, and the identity-matching identifiers when preparing a CHEMOS product inquiry.
相关技术资源
公开来源
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 化合物 10283713
PubChem · CID 10283713
常见问题
What does Chemical Phosphorylation Reagent II do?
It provides a phosphate group on an oligonucleotide chemically, during solid-phase synthesis, instead of using an enzyme (kinase). It couples like an amidite and, after deprotection, leaves a phosphate monoester — for example a 5'-phosphate.
How does it differ from the first Chemical Phosphorylation Reagent?
Both provide a terminal phosphate through the same protected-phosphate phosphoramidite concept. The 'II' name distinguishes a no-sulfur structure (C39H51N2O9P) from the sulfonyl-containing first reagent.
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