2'-Deoxyguanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
제품 개요
빠른 보기
- 제품군
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 분자식
- C44H54N7O8P
- 분자량
- 839.9
- 순도
- >=99% (HPLC, NMR)
2'-Deoxyguanosine (iBu) CE Phosphoramidite is the fully protected 2'-deoxyguanosine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65003 is identified by CAS 93183-15-4, molecular formula C44H54N7O8P, molecular weight 839.9, PubChem CID 135447702, and InChIKey FDRMKYVTIFSDPR-MMROLVBFSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the N2-isobutyryl base group is removed at final deprotection.
동의어
DMT-dG(iBu) phosphoramidite; N2-isobutyryl-5'-O-DMT-2'-deoxyguanosine 3'-CE phosphoramidite
식별 정보 및 규격
| Catalog No. | CH65003 |
| CAS No. | 93183-15-4 |
| Molecular Formula | C44H54N7O8P |
| Molecular Weight | 839.9 |
| PubChem CID | 135447702 |
| InChIKey | FDRMKYVTIFSDPR-MMROLVBFSA-N |
| Purity | >=99% (HPLC, NMR) |
보관 조건
-20 C
기술 속성
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Guanine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- N2-Isobutyryl
- Grade
- TheraPure; Standard grade
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; 210.1 g; 500 g; 1 kg; 10 kg; 20 x 40 g; 6 x 10 g; 6 x 20 g; Custom packaging on request
- Water content
- <=0.3%
- Coupling efficiency
- spec >=98.0%; observed 98.8%
- Physical form
- white to pale yellow powder
- Stability
- moisture sensitive; heat sensitive
응용 맥락
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
관련 기술 자료
공개 출처
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 화합물 135447702
PubChem · CID 135447702
자주 묻는 질문
What makes this a phosphoramidite building block?
It carries a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group — the reactive coupling group used in automated solid-phase DNA synthesis. It also has an acid-labile 5'-O-DMT group (removed each cycle) and an N2-isobutyryl base protection (removed at final deprotection).
How does it relate to 5'-O-DMT-N2-isobutyryl-2'-deoxyguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The 5'-O-DMT-N2-isobutyryl-2'-deoxyguanosine precursor is cataloged separately.
유사 제품
소재 선택에 도움이 필요하신가요?
CHEMOS는 제품 적합성, 목표 구조, 경로 이슈, 분석 기대치 및 프로젝트별 공급 요구를 검토합니다.