2'-Deoxyguanosine (dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
제품 개요
빠른 보기
- 제품군
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- 분자식
- C43H53N8O7P
- 분자량
- 824.9
- 순도
- 99.2% (HPLC)
2'-Deoxyguanosine (dmf) CE Phosphoramidite is the fully protected 2'-deoxyguanosine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-dimethylformamidine-2'-deoxyguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65006 is identified by CAS 330628-04-1, molecular formula C43H53N8O7P, molecular weight 824.9, PubChem CID 136334904, and InChIKey YRQAXTCBMPFGAN-UNHDIWNRSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the N2-dmf base group is removed at final deprotection.
동의어
DMT-dG(dmf) phosphoramidite; N2-dmf-5'-O-DMT-2'-deoxyguanosine 3'-CE phosphoramidite
식별 정보 및 규격
| Catalog No. | CH65006 |
| CAS No. | 330628-04-1 |
| Molecular Formula | C43H53N8O7P |
| Molecular Weight | 824.9 |
| PubChem CID | 136334904 |
| InChIKey | YRQAXTCBMPFGAN-UNHDIWNRSA-N |
| Purity | 99.2% (HPLC) |
보관 조건
-20 C
기술 속성
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Guanine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- N2-Dimethylaminomethylene (dmf)
- Grade
- Standard
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
- Water content
- K.F. <=0.20% w/w
- Coupling efficiency
- spec >=98.0% and observed 99.3%
- Physical form
- white to off-white powder
- Stability
- Specification print date 2026-01-01
응용 맥락
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- N2-dmf base protection: the dimethylformamidine group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
관련 기술 자료
공개 출처
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem 화합물 136334904
PubChem · CID 136334904
자주 묻는 질문
What makes this a phosphoramidite building block?
It carries a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group — the reactive coupling group used in automated solid-phase DNA synthesis. It also has an acid-labile 5'-O-DMT group (removed each cycle) and an N2-dimethylformamidine (dmf) base protection (removed at final deprotection).
How does it differ from 2'-deoxyguanosine (iBu) phosphoramidite?
Both are 5'-O-DMT 2'-deoxyguanosine phosphoramidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
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